Optimized Fluorescence Detection
Thank you for your interest in McPherson Fluorescence Detectors. Our customers say the FL-750 is the most versatile and sensitive fluorescence detector on the market! Fluorescence detection can be more complicated than absorbance. The much higher sensitivity of fluorescence detection usually determines when fluorescence is used in conjunction with or instead of absorbance. The McPherson FL-750 Fluoresce Detectors with application tuned light sources and high sensitivity accessories are in a class by themselves. See the NEW FL750 with integrated laptop computer control, our latest addition to this powerful line of instrument.
The McPherson FL-750 Fluoresce Detector permits signal to noise optimization for most applications. It can readily be configured for dynamic flow HPLC applications (FL-750B and BX versions) or used with cuvettes for static analysis (FL-750A.) The secret of the FL-750, and the key to optimization of detection, lies in the selection of the best excitation sources and dual or single monochromator detection modes.
The FL-750 fluorescence detector always contains two monochromators but the analyzer or emission monochromator can be bypassed when the High Sensitivity Filter Accessory (HSA) is used. This is the most sensitive method to detect emission. Calcium detection via emission monochromator and one HSA accessory permit simultaneous analysis of two emission wavelengths. Computer control of wavelength drives and data acquisition is possible. You can also do chemiluminescence and hydrocarbon detection with available accessories.
Excitation
| Light source selection has significant impact on the sensitivity in your application! One of the most important features of the F-750 is light source versatility. Three different CW light sources are available. Each has a characteristic emission spectrum and most effective wavelength area of use. These light sources are combined with a tunable excitation monochromator to allow efficient sample excitation at discrete wavelengths. Light sources include: Deuterium - for the low ultraviolet at or near 200-nm Xenon - general UV-VIS excitation Xenon-Mercury* - high performance excitation with intense Mercury lines * The Xenon-Mercury source is unique to McPherson. Xenon-Mercury is an ideal source for some applications and is favored because Fluorescence is directly proportional to the input excitation energy and indirectly influenced by background or noise. The high intensity Xenon-Mercury lines have low background and provide extremely high excitation energy. This results in excellent sensitivity and relatively strong emission energy even in cases where absorbance maximas are not a perfect match with the XeHg lines. |
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Emission
The FL-750 fluorescence detector efficiently collects emission spectra. The (usually) weak light emitted from the excited sample, can be collected two different ways. The user is free to select the mode of collection depending on the analytical or experimental requirement. Choose between two emission detection modes:
Monochromator - highest selectivity / continuously tunable wavelengths
Filter - highest sensitivity
Three Basic Scenarios in Fluorescence Detection
The FL-750 fluorescence detector is available in all the following instrument configurations. Select the best configuration given your analytical or experimental requirements.
Excitation wavelength is in the Ultraviolet at or close to 200-nm. The best combination of light source and monochromator instrument version is the following:
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Excitation in the UV-VIS, but not near mercury lines, 330-nm for example. The best combination of light source and monochromator instrument version is the following:
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Excitation at or near strong Mercury lines, 302 / 313, 365, 405 / 408 and 436-nm for example. Ultra sensitivity if the absorbance, and therefore emission maxima of the compound, is at or near a line.
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Optimization by Application
The FL-750 fluorescence detector is available in all the following instrument configurations. Select the best configuration given your analytical or experimental requirements.
| Application | Absolute Maxima | Excitation Range Used |
|---|---|---|
| Alflatoxins | 365 nm | 365 nm |
| DOPA | 365 nm | 365 nm |
| Folic Acid | 365 nm | 365 nm |
| Thiamine | 370 nm | 365 nm |
| Histamine | 360 nm | 365 nm |
| DEF | 365 nm | 365 nm |
| Actinomycin | 370 nm | 365 nm |
| Diphenhydramine | 365 nm | 365 nm |
| Penicillin | 365 nm | 365 nm |
| Aldosterone | 365 nm | 365 nm |
| Bile acids, NADH | 365 nm | 365 nm |
| P-aminosalicylic acid | 300 nm | 303 or 313 nm |
| Reserpine | 300 nm | 303 or 313 nm |
| Nitrosamines | 300 nm | 303 or 313 nm |
| Anthranilic acid | 300 nm | 303 or 313 nm |
| Salicylic acid | 310 nm | 313 nm |
| Pentothal | 315 nm | 313 nm |
| Thiopental sodium | 315 nm | 313 nm |
| Procaine hydrochloride | 310 nm | 313 nm |
| Chlorpromazine hydrochloride | 320 nm | 313 nm |
| Corticosteroids | 436 nm | 436 nm |
| Adriamycin** | 470 nm | 436 nm |
| Derivatives | ||
| Fluorescamine | 365 nm | 365 nm |
| Dansyl | 365 nm | 365 nm |
| Coumarin | 365 nm | 365 nm |
| Application | Absolute Maxima |
|---|---|
| Vitamin E | 205 nm |
| Eserine | 200 nm |
| Amino Acids | 229 nm (used with OPA) |
| Cocaine | 240 nm |
| Codeine | 245 nm |
| Phenobarbitol | 265 nm |
| Morphine sulfate | 285 nm |
| Hydrocarbons, oil | 210-250 nm |
| Albuterol | 225 nm |
| Application | Absolute Maxima |
|---|---|
| Vitamin E | 295 nm |
| Desmethylimipramine | 295 nm |
| Glutathione | 240 nm (used with OPA) |
| Parathione | 340 nm (used with OPA) |
| Tryptophan | 285 nm |
| Pyridoxine | 340 nm |
| Histidine | 340 nm |
| Vitamin K | 335 nm |
| DPNH | 340 nm |
| Vitamin A | 325 nm |
| Porphorins | 405 nm |